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KMID : 0903520090520030247
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 3 p.247 ~ p.251
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Antioxidation Effects of Rosmarinic Acid on Human Skin Melanoma Cells Treated with Hydrogen Peroxide
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Yoo Sun-Mi
Kang Jeong-Ran
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Abstract
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The purpose of this study was to evaluate the antioxidant effect of rosmarinic acid on the cultured human skin melanoma cells injured by reactive oxygen species. The cytotoxicity and antioxidant effect were analyzed by XTT assay for cell adhesion activity, DPPH-radical scavenging activity, lactate dehydrogenase (LDH) activity after treated of cells with or without hydrogen peroxide (H2O2) for 1-10 h. H2O2 decreased the cell adhesion activity in cultured human melanoma cells in a dose- and time-dependent manner after cells were treated with 20-110 μM of H2O2 for 5-7 h. IC50 values of H2O2 by XTT assay were 90 μM, respectively. H2O2 increased LDH activity dose dependently. LDH50 was determined at 60 μM H2O2 after treatment of cells with 50-90 μM H2O2 for 8 h. Rosmarinic acid increases cell adhesion activity, DPPH-radical scavenging and decreases LDH activity. The treatment of the cells with H2O2 shows highly toxic effects on cultured human skin melanoma cells. Rosmarinic acid increases cell adhesion activity, DPPH-radical scavenging activity and decreases LDH activity in the cells treated with H2O2. It is considered that rosmarinic acid shows antioxidant effect on ROS such as H2O2.
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KEYWORD
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DPPH-radical scavenging, LDH, rosmarinic acid, XTT assay
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